Coding

Part:BBa_K2598011:Design

Designed by: Matthew Gao   Group: iGEM18_UCAS-China   (2018-09-22)


T7 RNAP sigma fragment CGG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 578
    Illegal NgoMIV site found at 1193
    Illegal NgoMIV site found at 1202
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

It's obtained by Segallshapiro T H et al

References

Segall-Shapiro, T. H., Meyer, A. J., Ellington, A. D., Sontag, E. D. & Voigt, C. A. A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol 10, 742, (2014)

Fernandez-Rodriguez, J., Moser, F., Song, M. & Voigt, C. A. Engineering RGB color vision into Escherichia coli. Nature Chemical Biology 13, 706-708 (2017)